Monoclonal antibody detection of prolactin-binding subunits in the rabbit mammary gland
نویسندگان
چکیده
منابع مشابه
Biological activities of binding site specific monoclonal antibodies to prolactin receptors of rabbit mammary gland.
The biological activity of three monoclonal antibodies (mAbs) against the rabbit mammary prolactin (PRL) receptor (M110, A82, and A917) were investigated using explants of rabbit mammary gland. The three mAbs which were all able to inhibit the binding of 125I-ovine prolactin to its receptor had different biological activities. Two mAbs (M110 and A82) were able to prevent the stimulating effect ...
متن کاملTwo separate receptors for prolactin in the rabbit mammary gland.
Rabbit mammary gland PRL receptors in the microsome fraction were solubilized with the zwitterionic detergent Chaps, and were separated into two fractions (Fr. A and B) by ion-exchange chromatography. The number of receptors in Fr. B was about 2.2 times greater than in Fr. A. In sucrose gradient centrifugation analysis, PRL receptors in Fr. A and Fr. B sedimented at different positions. After b...
متن کاملProperties of a prolactin receptor from the rabbit mammary gland.
Receptors for human, simian, ovine, bovine and murine prolactin, human growth hormone and human placental lactogen have been identified in plasma-membrane-containing subcellular particles isolated from rabbit mammary glands. The association and dissociation of (125)I-labelled prolactin are time- and temperature-dependent processes, both being maximal at 37 degrees C. (125)I-labelled prolactin p...
متن کاملThermodynamic analysis of the interaction of prolactin with its receptor in the rabbit mammary-gland microsomes.
The interaction of prolactin (PRL) with its membrane receptor depends markedly on temperature. Thermodynamic parameters for this reaction have been evaluated from data for time-course kinetics and equilibrium binding at multiple temperatures between 19 and 31 degrees C. The free-energy change with temperature and the van't Hoff plot were found to be linear. These suggest that there are minimal ...
متن کاملIdentification of prolactin-induced transcription factors in the mammary gland
a number of biological processes in vertebrates, including reproduction, growth and development, osmoregulation, metabolism and immunoregulation (Nicoll, 1974). The biological actions of prolactin have been most extensively characterized in the mammary gland, where it has an essential role both in the differentiation of the gland during pregnancy and in the regulation of milk protein gene expre...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1988
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2560917